mouse il23  (R&D Systems)

Journal: bioRxiv

Article Title: Allosteric inhibition of CXCR1 and CXCR2 abrogates Th2/Th17-associated Allergic Lung Inflammation in Mice

doi: 10.1101/2024.05.13.593638

Figure Lengend Snippet: ( A-B ) Il23, Il1b, and Il6 mRNA expressions in the BALF cells ( A ) and in the lung ( B ) in CDE-MCM at 28 h post-CDE challenge. ( C ) The protein levels of Il23 in the lung in CDE-MCM at 28 h post-CDE challenge. ( D ) The numbers of IL23 producing T-cells, neutrophils, eosinophils, and epithelial cells in the lung in CDE-MCM at 72 h post-CDE challenge. BALF; bronchoalveolar lavage fluid CDE; cat dander extract CDE-MCM; CDE-multiple challenge model Th17; T helper 17

Article Snippet: Lung single cells were pre-incubated with TruStain FcX solution (BioLegend, San Diego, CA) for 10 mins at 4°C, and stained with fluorophore-conjugated antibodies Fixable Viability Dye eFluor 780 (eBioscience, San Diego, CA), CD45 monoclonal antibody-pacific orange (Thermo Fisher Scientific, MA, USA, #MCD4530, clone 30-F11), anti-mouse CD3-Alexa Fluor 700 (Biolegend, #100216, clone 17A2), rat anti-mouse CD4-Brilliant Violet 520 (BD Biosciences, #563106, clone RM4.5), PE rat anti-mouse CD181 (CXCR1) (BD Biosciences, San Jose, CA, #566383, clone U45-632), BV711 Rat Anti-Mouse CD182 (CXCR2), (BD Biosciences, #747812, clone V48-2310), BV786 rat anti-mouse Siglec-F (BD Biosciences, #740956, clone E50-2440), Spark YG 593 anti- mouse Ly-6G Antibody (Biolegend, #127668, clone 1A8), Spark NIR™ 685 anti-mouse/human CD11b Antibody (Biolegend, #101278, clone M1/17) at 1:100 dilution in flow stain buffer containing FBS for 30 mins at 4°C, then cells were permeabilized with Fixation/Permeabilization kit (BD Biosciences) according to the protocol from vendor and stained with intracellular cytokine specific antibodies Rat anti-mouse Rorγt- Brilliant Violet-650 (BD Biosciences, #564722, clone Q31-378), mouse anti-GATA3 Alexa Fluor F488 (BD Bioscience #560163, clone L50-823,) Rat anti-mouse IL4-PE/Cyanine7 (BD Biosciences, #560699, clone 11B11), anti-mouse/anti-human IL5-Allophycocyanin (APC) (BD Biosciences, #554396, clone TRFK5), IL13 monoclonal antibody ((eBio13A), Brilliant Ultra Violet 805, eBioscience), Rat Anti-Mouse IL17A-PE (BD Biosciences, # 559502, clone TC11-18H10), and rat anti-mouse IL23 p19 Alexa Fluor 647 (BD Biosciences, # 565317, clone N71-1183) for 45 mins at 4°C.

Techniques:

Journal: bioRxiv

Article Title: A Novel human IL-23A Overexpressing Mouse Model of Systemic Lupus Erythematosus

doi: 10.1101/2023.11.08.566163

Figure Lengend Snippet: (A) Schematic representation of the human IL23A transgene . (B) human IL23A mRNA detected by PCR and (C) human IL23A protein detected by immunohistochemistry in the skin, cervical lymph nodes, spleen, lung and kidney of TghIL23A mice. Scale bar 200μm and 100μm.

Article Snippet: Tissue paraffin sections were stained with H&E for histopathological evaluation or with biotinylated anti-mouse-IgG (Vector; BA-9200) for the detection of immune complex deposits and anti-human IL23A (OriGene; AM20386PU-N) for the detection of human IL23A protein in various tissues.

Techniques: Immunohistochemistry

Journal: bioRxiv

Article Title: A Novel human IL-23A Overexpressing Mouse Model of Systemic Lupus Erythematosus

doi: 10.1101/2023.11.08.566163

Figure Lengend Snippet: Reduced skin (upper panel) and ear (lower panel) clinical (A) and histopathological signs (B), as well as reduced LN/BW and Spleen/BW ratios (C) , urine protein levels (D) IgG circulating levels (E) and glomerular IgG deposits (F) following a 10-week-long treatment of TghIL23A mice with anti-human IL23A (Guselkumab:GUS). Scale bar 20μm. Data are presented as mean ± SEM; * P < 0.04; ** P < 0.0092; *** P = 0.0002; **** P < 0.0001.

Article Snippet: Tissue paraffin sections were stained with H&E for histopathological evaluation or with biotinylated anti-mouse-IgG (Vector; BA-9200) for the detection of immune complex deposits and anti-human IL23A (OriGene; AM20386PU-N) for the detection of human IL23A protein in various tissues.

Techniques: